rabbit polyclonal antibodies against lyve-1  (Santa Cruz Biotechnology)

Journal: The British Journal of Ophthalmology

Article Title: Tumour-associated lymphangiogenesis in conjunctival malignant melanoma

doi: 10.1136/bjo.2008.147355

Figure Lengend Snippet: Tumour-associated lymphatic vessels (LVs) in malignant melanomas (MMs) of the conjunctiva. (A) Representative image of LV staining with LYVE-1 antibody as specific marker for lymphatic endothelium: LYVE-1 positive peritumoural LVs. (B) Representative image of podoplanin stained LVs in the tumour adjacent conjunctiva. (C) Intratumoural LYVE-1 positive LVs (magnification ×100/×200). The area of primary acquired melanosis is marked with an asterisk. Arrows denote the LYVE-1/podoplanin stained lymphatic vessels. Note that erythrocyte-filled blood vessels are not stained with these lymphatic endothelial specific markers. LU, lymphatic vessel lumen.

Article Snippet: Sections of conjunctival MMs were incubated for 30 min with a rabbit polyclonal antibody against human LYVE-1 (1:100; Dako, Hamburg, Germany) and HRP-conjugated secondary antibody before development with 3-amino-9-ethylcarbazole (AEC + ) substrate (red reaction product) or 3,3′-diaminobenzidine (DAB; brown product).

Techniques: Staining, Marker

Journal: The British Journal of Ophthalmology

Article Title: Tumour-associated lymphangiogenesis in conjunctival malignant melanoma

doi: 10.1136/bjo.2008.147355

Figure Lengend Snippet: Tumour-induced lymphangiogenesis. Significantly more proliferating lymphatic vessels (LVs) were found intratumourally and next to the tumour than in distant conjunctiva (>300 μm). Representative images of conjunctival malignant melanoma specimen stained with LYVE-1 and proliferation marker Ki67. Ki67 positive cells are marked (arrowhead). (A) Representative image of Ki67 positivity in tumour-associated lymphatic endothelial cells; red: Ki-67; brown: LYVE-1 (magnification ×1000). (B) Significantly more proliferating LVs were found intratumourally as well as in the directly adjacent tumour environment than in the more distant conjunctiva. A paired t test was performed for each zone separately with the following results: (1) tumour versus tumour adjacent conjunctiva (50 μm zone) p = 0.063 (not significant); (2) tumour versus mid-peripheral zone (50–200 μm) p = 0.021; (3) tumour versus peripheral zone (200–300 μm) p = 0.031; (4) tumour versus conjunctiva >300 μm from the tumour border p = 0.002; n = 20, median is marked; circles mark three outliers. The results support the hypothesis of tumour-associated active lymphangiogenesis in the proximity of the tumour.

Article Snippet: Sections of conjunctival MMs were incubated for 30 min with a rabbit polyclonal antibody against human LYVE-1 (1:100; Dako, Hamburg, Germany) and HRP-conjugated secondary antibody before development with 3-amino-9-ethylcarbazole (AEC + ) substrate (red reaction product) or 3,3′-diaminobenzidine (DAB; brown product).

Techniques: Staining, Marker